Cisplatin ic50 sh-sy5y

The neuroblastoma cell line SH-SY5Y, obtained fromthe Shanghai Institute of Biochemistry and Cell Biology (Shanghai,China), was cultured in DMEM/F12 (1:1) (HyCLone, Logan, UT, USA)with 10% FCS (HyClone), 100 U/ml penicillin and 100 μg/mlstreptomycin at 37°C in 5% CO2. Cells were detached … See more Neuroblastoma is one of the most common childhoodtumors, usually diagnosed at a median age of 17 months (1). The tumor originates from neural-cresttissues, deep in … See more To select an effective concentration of cisplatinfor suppressing the proliferation of SH-SY5Y cells, an MTT assaywas performed to … See more The present study was supported by the NCET-10-0919,National Natural Science Foundation (nos. 31371321, 81200601), theShandong Science and Technology Committee (no. ZR2012HQ035), theFoundation of … See more BDNF, initially identified as a neurotrophin, playsan essential role in a variety of neuronal functions, includingproliferation, differentiation and survival in the central nervoussystem (CNS). It also contributes to the regulation of … See more Webcomplexes 2 and 3 resulting in greater cell uptake than cisplatin in selected tumor cell lines, only 3 showed comparable or higher antitumor activity. General low cytotoxicity of complex 2 in the tested cell lines (SH-SY5Y, SK-OV-3, Hep-G2, Caco-2, HeLa, MCF-7, MG-63, ZL-65) appeared to depend

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WebOct 30, 2012 · SH-SY5Y cells were seeded in 6-well plates (at 2×10 6 /ml) and incubated for 24 h until they were treated with 0.1 μM VCR for 6, 12, 18 and 24 h. Non-treated cells at 0 h were used as the control group. Cells were washed twice with ice-cold PBS and fixed with 70% ice-cold ethanol. WebSH-SY5Y were deposited to the ATCC(®) in 1970 by June L. Biedler.Three important characteristics of SH-SY5Y cells should be considered when using these cells in in vitro studies. First, cultures include both adherent and floating cells, both types of … pornography in india wiki https://bennett21.com

SH-SY5Y - CRL-2266 ATCC

WebOct 23, 2024 · Cisplatin 5 µg and different concentrations (Cisplatin + CoS NPs bioconjugates (5, 10, 25, 50, 75 μg) doses were applied to SH-SY5Y neuroblastoma cell … WebIC50 values for (a-d) bortezomib-and (e-h) cisplatin-treated MCF7, HCC38, MCF-10A, and MDA-MB-436 breast cancer cells generated in the present study and pharmacoDB 42 datasets. No bar is shown... WebSep 23, 2016 · The changing 50% inhibitory concentration (IC50) of cisplatin: A pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer... sharp optonica cp-2525h

Reverse microscope image cisplatin-MgS bioconjugate NPs on …

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Cisplatin ic50 sh-sy5y

Real-time monitoring of cisplatin cytotoxicity on three …

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Cisplatin ic50 sh-sy5y

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WebThe IC50 values of the two platinum derivatives varied considerably. The values for cisplatin varied between 0.022 microgram ml-1 and 0.56 microgram ml-1 and the corresponding values for carboplatin were 0.096-1.20 microgram ml-1. The range of the ratios between carboplatin IC50 and cisplatin IC50, from 1.5:1 to 4.4:1, was rather narrow. WebOct 25, 2016 · The changing 50% inhibitory concentration (IC50) of cisplatin: a pilot study on the artifacts of the MTT assay and the precise measurement of density-dependent chemoresistance in ovarian cancer Oncotarget 2016 Oct 25;7 (43):70803-70821. doi: 10.18632/oncotarget.12223. Authors

WebDrug: Cisplatin - Cancerrxgene - Genomics of Drug Sensitivity in Cancer Genomics of Drug Sensitivity in Cancer Compound: Cisplatin Drug Target: DNA crosslinker Drug Target … WebSep 15, 2024 · Cisplatin is a prescription medicine used to treat the symptoms of cancer in the testicles (Metastatic Testicular Tumors), bladder (Advanced Bladder Cancer) and …

WebNov 2, 2024 · IC 50 values for single methotrexate treatments were 1.2 µM (R 2 = 0.9624) for MCF-7, and 0.8 µM (R 2 = 0.9583) for SH-SY5Y cells (Fig. 1 ). Therefore, combination doses of methotrexate were calculated and used according to these values. WebFeb 16, 2024 · Generic name: cisplatin [ sis-PLA-tin ] Brand names: Platinol, Platinol-AQ, Platinol (Restricted Access) Dosage forms: intravenous powder for injection (50 mg), …

WebOct 1, 2024 · The purpose of this study was to evaluate the effect of 50-Hz electromagnetic field (EMF) in combination with CDDP and bleomycin (Bleo) on expression of some of DNA repair genes (GADD45A, XRCC1,...

WebMar 31, 2016 · View Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn … sharp optonica sm 110WebSensitivity testing results, expressed as the attention of medicine called for to prevent by 50 % the development of infection in cell society (IC50), differ substantially relying on a … sharp order musicWebwhich ranks it as about average compared to other places in kansas in fawn creek there are 3 comfortable months with high temperatures in the range of 70 85 the most ... pornography issues in the philippinesWebIn the present study, we observed the effect of cisplatin on BDNF expression in SH-SY5Y cells and investigated the mechanism of cisplatin in inducing the apoptosis of SH-SY5Y … sharp optometry sorrento mesaWebAn IC50 curve for MCF7 cells treated with cisplatin. Percentage cell survival is plotted against the logarithm of treatment concentrations. Data points are the means±SD of duplicate... pornography is of concern to:WebFrom the 35 % solution (probably in water) prepare a working stock in water and dilute it in PBS to get the designated final concentration (s); treat the cells H2O2 in PBS for 10-20 min; analyze... sharp optonica st-5200WebThe IC25 and IC50 values of NaF for 3, 12 and 24 h and optimum dose of increase in proliferation to vitamin D3 through MTT assay in NRK-52E kidney cells were determined. DNA damage was significantly increased (p < 0.05) compared to the control group in all groups except for vitamin D3. pornography laws by country