Ntf2 mediates nuclear import of ran
Web17 jun. 2009 · RAN (Ras-related nuclear protein) is a small GTP-binding protein belonging to the RAS superfamily (see 190020) that is essential for the translocation of RNA and proteins through the nuclear pore complex ( Ren et al., 1993 ). The RAN protein is also involved in control of DNA synthesis and of cell cycle progression. Web16 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct …
Ntf2 mediates nuclear import of ran
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WebRan is a small G protein that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein has also been implicated in the control of DNA synthesis and cell cycle progression, as mutations in Ran have been found to disrupt DNA synthesis. [8] Function [ edit] Ran cycle [ edit] Webthe docking of NLS-proteins at the nuclear pore complex (NPC). RanT24N, in contrast to wild-type Ran-GDP, interacts only weakly with importin α and nucleoporins, and not at all with the import factor p10, consistent with its poor activity in nuclear import. However, RanT24N does interact stably with importin β, Ran binding protein 1 and RCC1 ...
Webtemperature-sensitive NTF2 mutants are defective in nuclear protein import. Moreover, temperature sensitive mutants of the yeast Ran homolog Gsplp can be suppressed by overexpression of NTF2, sug gesting that Ran-NTF2 interaction is required for effective nuclear protein import in vivo (Wong et al., 1997). Whether NTF2 is also required for … Web1 dec. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2-RanGDP complex to the nuclear side of the NPCs. A direct …
WebThe experiment shows that this mutant Ran failed to accumulate in the nuclei, confirming that the direct NTF2–RanGDP interaction is crucial for Ran import. In addition, we can conclude that... Web1 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2-RanGDP complex to the nuclear side of the NPCs. A direct …
WebNuclear import in digitonin-permeabilized cells in the absence of added factors was minimal. The addition of energy and nuclear transport factor 2 (NTF2) [5] was sufficient for the accumulation of Ran in the nucleus. An NTF2 mutant that cannot bind Ran [6] was unable to facilitate Ran import. A GTP-bound form of a Ran mutant that cannot bind ...
WebNTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct NTF2–RanGDP … englewood beach yarmouth maWebNuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP. The simplicity and specialization of this system, combined with the availability of crystal structures of NTF2, RanGDP and their complex, has facilitated the investigation of the … dreamweaver activationWebis recognized in the cytoplasm by NTF2 (nuclear transport fac- tor 2), which mediates its rapid translocation into the nucleus where the Ran guanine nucleotide exchange factor RCC1 (regu- dreamweaver activation codeWeb18 jan. 2002 · We also used a mutant species [Ran(T24N)], which is defective in binding to nuclear transport factor 2 (NTF2), the Ran import carrier (4, 13, 14). The rate of nuclear accumulation of FL-Ran(T24N) was <4% that of wild-type FL-Ran (Fig. 1B) and was similar to the rate of diffusion of green fluorescent protein (GFP) into the nucleus (15,16). englewood buy sell tradeWebRibbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1998) NTF2 mediates nuclear import of Ran. EMBO J 17, 6587–6598. CrossRef PubMed CAS Google Scholar Ribbeck K, Kutay U, Paraskeva E, Görlich D (1999) The translocation of transportin-cargo complexes through nuclear pores is independent of both Ran and energy. dreamweaver 8 english versionWeb16 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct … dreamweaver activityWebNTF2 has a much lower affinity for RanGTP than RanGDP, and thus travels empty back to the cytoplasm after its cargo (RanGDP) is converted to RanGTP by RCC1 inside the nucleus triggering its release. This constant import of Ran by NTF2 into the nuclear interior keeps Ran concentrated inside the nucleus. View chapter Purchase book Myogenesis dreamweaver adobe dreamweaver