Webb14 nov. 2024 · As expected, immunoblotting (IB) assays revealed that OGT was clearly detected in the co-immunoprecipitates obtained with the anti-SIRT1 antibody but not … Webb9 mars 2016 · In vitro UDP-Glo assay. In this work OGT activity was also determined using UDP-Glo ™ Glycosyltransferase Assay according to the manufacturer’s …
O-GlcNAc transferase couples MRE11 to transcriptionally active ...
Webb8 aug. 2016 · Low OGT activity reduces O-GlcNAcylation and promotes c-MYC degradation to maintain cell survival. In the presence of glucose, PP1γ-bound URI increases OGT and c-MYC levels. Accordingly, mice expressing non-phosphorylatable URI (S371A) in hepatocytes exhibit high OGT activity and c-MYC stabilization, accelerating … WebbOGA Activity Assays Celllysatesdesaltedinto20-mMTrisHCl,pH7.8,20%(v/v)glycerol (asdescribedabove)wereassessedforOGAactivityin50-mMsodium cacodylate, pH 6.4, 0.1 mg/ml BSA, and 100-mM GalNAc using fluo- rescent substrates (1-mM): 4-methylumbelliferyl (4-MU)–GlcNAc or 4- MU–GalNAc (MilliporeSigma) (16, 27). β-N … nourish skin tight firming lotion
Cisplatin enhances protein O ‑GlcNAcylation by altering the activity …
WebbInterdisciplinary scientist in chemical and structural biology with expertise in synthetic chemistry, enzymology, assay development, glycobiology … Webb19 juni 2015 · O-GlcNAc transferase (OGT) is an essential mammalian enzyme that regulates numerous cellular processes through the attachment of O-linked N … WebbMethods in Molecular Biology, Springer / Caroline Smet-Nocca (Ed.) janv. 2024. This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology. Divided into five parts that illustrate the underlying molecular mechanisms of Tau ... nourish skin ultimate