Phleomycin plates
Webb1 feb. 2002 · Selection on phleomycin plates gave rise to resistant fungal colonies only when transformed A. tumefaciens was used for cocultivation together with AS, indicating that induction of the vir-genes was essential for T-DNA transfer between A. tumefaciens and hyphae of ecto- mycorrhizal fungi. WebbThe working concentration of phleomycin for mammalian cell lines varies from 5 to 50 μg/ml. In a starting experiment we recommend to determine the optimal concentration …
Phleomycin plates
Did you know?
Webb1 jan. 2012 · GYE agar plates containing 3 μg/mL of phleomycin (GYE-Phl): Prepare and autoclave 500 mL GYE agar as described in Subheading 2, item 1. Add 0.5 mL of 3 mg/mL phleomycin stock solution into the warm GYE agar and dispense 10 or 20 mL into each 60- × 15-mm or 100- × 15-mm Petri plate, respectively.
Webb19 sep. 2013 · The pretreated MA plates were prepared by adding either 50 or 100 µg of Phleomycin (from a 20mg/mL stock) drop wise to each plate in three different regions. After allowing the antibiotic to dry (c.a., 30 min), the individual strains were plated across an entire plate. Resistance was evaluated after two days of growth at either 25°C or 13°C. WebbTo isolate a homokaryotic strain, we perform multiple subcultures of the single colonies which contain heterokaryotic cells on selection plates with hygromycin B or phleomycin to enrich for the mutated nuclei. Homokaryotic mutants can be obtained after three to four subcultures of isolated colonies.
Webbhygromycin B and phleomycin were purchased from Cayla. For efficient selection, some batches of hygromycin B required higher concentrations (up to 400 µg/ml). Phleomycin … Webb1 maj 1996 · agar plates in the absence of phleomycin for more than. 18 months and retain resistance to the drug at 2 l g/ml. DNA was isolated from the six Pm R transformants …
WebbBleomycin and phleomycin are closely related broad-spectrum antibiotics produced by strains of Streptomyces verticillus; they are effective against both prokaryotic and …
WebbFig.2 Phleomycin test plates (1-3: 0, 25, 50 ug/mL) As the images shown above, the growing situation of wild type strain M. anisopliae on hygromycin or phleomycin plates … can i change my last name before marriageWebb5-alpha Competent E. coli cells were transformed with 2 µl of the reaction sample following the Gibson Assembly Transformation Protocol from NEB. Next, 100 µl of the cells were plated onto LB medium plates with Phleomycin at a concentration of 20 ug/ml. The plates were incubated overnight at 37 °C. Figure 9. fitness watch that doesn\u0027t need smartphoneWebbPhleomycin, 2% solution in water; Signal Word. Warning; Hazard Category. Carcinogenicity Category 2; Respiratory sensitiser Category 1; Hazard Statement. H351-Suspected of … fitness watch where to buyWebbSerial dilutions of each strain were plated onto YEPD, MMS plates (0.003% and 0.004%), and phleomycin plates (3 g/ml and 6 g/ml). To measure UV sensitivity, ... can i change my laptop cpuWebbChemical structure of phleomycin D1. Phleomycins are a group of glycopeptide antibiotics found in Streptomyces which are closely related to bleomycin. [1] Examples include: … can i change my lawyerWebbPhleomycin, supplied by BioShop, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more can i change my last name back to maiden nameWebbPhleomycin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS ... °C and number of cells were normalized to … can i change my legal name online